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May 03
Fresh hope for infertile men
A new study has found that stem cells made from the skin of adult, infertile men yield primordial germ cells - cells that normally become sperm - when transplanted into the reproductive system of mice.

The infertile men in the study each had a type of genetic mutation that prevented them from making mature sperm - a condition called azoospermia.

The research at the Stanford University School of Medicine and Montana State University suggests that the men with azoospermia may have had germ cells at some point in their early lives, but lost them as they matured to adulthood.

Although the researchers were able to create primordial germ cells from the infertile men , their stem cells made far fewer of these sperm progenitors than did stem cells from men without the mutations.

The research provides a useful, much-needed model to study the earliest steps of human reproduction.

"We saw better germ-cell differentiation in this transplantation model than we've ever seen," Renee Reijo Pera, PhD, former director of Stanford's Center for Human Embryonic Stem Cell Research and Education said.

"We were amazed by the efficiency. Our dream is to use this model to make a genetic map of human germ-cell differentiation, including some of the very earliest stages," Pera said.

The research is set to be published in Cell Reports.

May 03
Not mandatory for private hospitals to treat poor for free: Delhi HC
In a setback to poor patients, the Delhi High Court Monday exempted city's four big private hospitals from the "mandatory obligation" to provide free treatment to certain percentage of poor patients.

Saying such mandatory requirement was "not provided in their lease deed", a division bench of Justice S.Ravindra Bhat and Justice R.V.Easwar allowed the pleas of the hospitals challenging the central government's order of incorporating a condition inserted in its lease deed for free treatment to the poor.

The government had mandatory for private hospitals, built on land allocated by the government on concessional rates, to provide 10 percent treatment to poor people in the patient department (IPD) and 25 percent in the out patient department (OPD).

The bench order came on a bunch of petitions filed by four hospitals - Mool Chand Khairati Ram Trust, St.Stephen's Hospital, Sitaram Bhartia Institute of Science & Research and Foundation for Applied Research in Cancer - challenging the central government's order on the ground that it was not part of the conditions of lease deeds.

The central government had contended that providing treatment to the poor was one of the clause in the lease deed due to which land was given to hospitals at a very cheap price.

In its judgment, the court said: "What the government is seeking to achieve is untenable," adding that "neither the allotment nor the lease deed contained any requirement to provide free medical facilities" and submission of the central Delhi government that they had the power to issue direction in the regard are "untenable".

May 02
Vitamin D deficiency linked to aggressive prostate cancer
Lately, there's been a lot of controversy about Vitamin D recommendations, which in turn has led many to puzzlement on how much they should be taking.

Despite this great disagreement among experts and scientists, Vitamin D deficiency has now been linked to aggressive prostate cancer, according to a new study.

The study hints that Vitamin D may play a crucial role in how prostate cancer starts and spreads, although this does not prove a cause-and-effect relationship.

"Vitamin D deficiency could be a bio-marker of advanced prostate tumour progression in large segments of the general population," said Adam B. Murphy, Northwestern University's Feinberg School of Medicine.

He said it would be wise for men to be screened for Vitamin D deficiency and treated although more research is needed.

Rick Kittles, an associate professor in the department of medicine at University of Illinois at Chicago (UIC) said: "This is the first study to look at Vitamin D deficiency and biopsy outcomes in men at high risk of prostate cancer".

In the study, scientists examined data collected from more than 600 men between the ages of 40 and 79 from the Chicago area who had elevated prostate-specific antigen (PSA) levels or other risk factors for prostate cancer.

Each man was screened for Vitamin D deficiency before undergoing a prostate biopsy.

The authors were surprised to find that Vitamin D deficiency seemed to be a predictor of aggressive forms of prostate cancer diagnosis in African-American and European-American men.

"These men, with severe Vitamin D deficiency, had greater odds of advanced grade and advanced stage of tumours within or outside the prostate," Murphy added.

Unless it is severe, Vitamin D deficiency is fairly asymptomatic, so more effort needs to be put on screening.

The study has been published in the journal Clinical Cancer Research.

May 02
Antibiotic resistance serious global threat to public health: WHO
The World Health Organisation (WHO) has warned in its new report that resistance to antibiotics poses a serious global threat to public health.

WHO's new report (its first to globally look at antimicrobial resistance, including antibiotic resistance) reveals that this grave threat is no longer a prediction for the future but is happening right now in every region of the world and has the potential to affect anyone, of any age, in any country.

It depicted "post-antibiotic era", where people die from simple infections that have been treatable for decades.

"Without urgent, coordinated action by many stakeholders, the world is headed for a post-antibiotic era, in which common infections and minor injuries which have been treatable for decades can once again kill," says Keiji Fukuda, WHO's assistant director-general for health security.

"Effective antibiotics have been one of the pillars allowing us to live longer, live healthier, and benefit from modern medicine. Unless we take significant actions to improve efforts to prevent infections and also change how we produce, prescribe and use antibiotics, the world will lose more and more of these global public health goods and the implications will be devastating," he added.

The report focuses on seven different bacteria responsible for common, serious diseases such as bloodstream infections (sepsis), diarrhoea, pneumonia, urinary tract infections and gonorrhoea.

The results are worrisome as the so-called "last-resort" antibiotics no longer work in more than half of people being treated in countries.

While bacteria naturally mutate to eventually become immune to antibiotics, the misuse of these drugs - such as doctors over-prescribing them and patients failing to finish courses has worsened the crisis.

Urging public to take simple precautions such as washing hands to prevent bacteria from spreading in the first place, WHO's report called for better access to clean water, infection control in healthcare facilities, and vaccination - to reduce the need for antibiotics.

WHO is also calling attention to the need to develop new diagnostics, antibiotics and other tools to allow healthcare professionals to stay ahead of emerging resistance.

This report is kick-starting a global effort led by WHO to address drug resistance.

This will involve the development of tools and standards and improved collaboration around the world to track drug resistance, measure its health and economic impacts, and design targeted solutions.

Antibiotics are medicines that either kill or inhibit the growth of a bacteria, but they cannot cure everything. Different types of antibiotics are there and each works a little differently, acting on different types of bacteria.

May 01
Insulin-producing cells derived from cloned human embryo
In a pioneering research involving stem cells, researchers in the US have successfully used a cloning technique to make insulin-producing cells with the DNA of a diabetic woman.

A team led by regenerative medicine specialist Dieter Egli at the New York Stem Cell Foundation Research Institute derived embryonic stem cells from a cloned embryo containing the DNA from a 32-year-old woman with type 1 diabetes.

The researchers also succeeded in differentiating these ES cells into insulin-producing cells - opening a new pathway for treating diabetes by replacing pancreatic cells.

"The new work is a step toward providing genetically matched replacement cells for transplant," Egli said.

To produce the cloned embryos, the researchers used an optimised version of the laboratory technique called somatic-cell nuclear transfer (SCNT).

In the technology, the nucleus from a patient's cell is placed into an unfertilised human egg which has been stripped of its own nucleus.

This reprogrammes the cell into an embryonic state.

SCNT was the technique used to create the first mammal cloned from an adult cell - Dolly the sheep - in 1996.

The eggs were grown into early embryos.

From these, the scientists removed stem cells, which can grow into any cell type in the body.

The scientists turned these stem cells into the insulin-producing cells.

According to researchers, the breakthrough is a step toward providing perfectly genetically matched replacement cells for transplant.

In a previous research this month, researchers led by Young Gie Chung and Dong Ryul Lee at the CHA University in Seoul reported in Cell Stem Cell that they had cloned embryonic stem-cell (ES cell) lines made using nuclei from two healthy men, aged 35 and 752.

The studies show that the technique works for adult cells and in multiple labs, marking a major step.

"It's important for several reasons," said Robin Lovell-Badge, a stem-cell biologist at the MRC National Institute for Medical Research in London.

The research has been published in the journal Nature.

May 01
Breath analysis could help detect early lung cancer
Researchers at the University of Louisville School of Medicine are now using breath analysis to detect the presence of lung cancer.

Preliminary data indicate that this promising noninvasive tool offers the sensitivity of PET scanning, and has almost twice the specificity of PET for distinguishing patients with benign lung disease from those with early stage cancer.

Michael Bousamra II, MD, Associate Professor, Department of Cardiovascular and Thoracic Surgery, presented the results of the study at the AATS 2014 Conference on April 29, 2014.

A reliable, noninvasive diagnostic method imposes less physical and financial burden on patients who actually have no significant disease, while rapid and accurate diagnosis expedites treatment for patients who truly have lung cancer.

The team believes that while the breath test would not replace CT as a primary screening tool, it would be particularly helpful in conjunction with a positive CT scan result.

"This breath analysis method presents the potential for a cheaper and more reliable diagnostic option for patients found to have bulky disease on a CT scan . If the breath analysis is negative, the patient may, in some instances, be followed with repeated exams without necessitating a biopsy. But a positive breath analysis would indicate that the patient may proceed to definitive biopsy, thus expediting treatment," Dr. Bousamra said.

Apr 30
People living in wealthy countries more stressed out: Study
A new study claims that people living in wealthy countries experience more stress and anger than those who live in poorer countries.

People living in a well-off country might be more satisfied in their lives but that can make them more stressed out.

"Life in an affluent country is more fast-paced and there are just so many things that you have to do leading to stress," Louis Tay, an assistant professor of psychology at Indiana-based Purdue University, was quoted as saying.

To carry out the study, the researchers examined income data from nearly 840,000 people in 158 nations.

Participants reported how satisfied they were with their lives and whether they experienced various feelings such as "worry," "sadness" and "anger", a LiveScience report said.

There is a link between anxiety and having too many choices because trying to make the best choice fast can stress people out.

Researchers also found that people who lived in wealthier countries expressed higher levels of life satisfaction than those who made a similar amount of money, but lived in poorer countries.

The study has been published in the journal Psychological Science.

Apr 30
Kids' waist circumference can predict metabolic disease risk: Study
Attention parents, the circumference of your child's waist can now predict if he or she is likely to suffer from metabolic diseases.

Now, a multi centre cross-sectional study carried out by the International Diabetes Federation hints that healthy cut off values of waist circumferences can be used as screening for Metabolic Syndrome (MS) in Indian children.

The study was conducted on 10,842 children in five cities - Delhi, Chennai, Kolkata, Pune and Raipur.

"MS in children has been defined as the presence of high triglyceride levels in blood, Low HDL cholesterol (good cholesterol), increased fasting blood glucose levels, high systolic blood pressure and waist circumference > 75th percentile," said Dr Archana Dayal Arya, Paediatric Endocrinologist of Sir Ganga Ram Hospital, New Delhi and co-author of the study.

Metabolic Syndrome results in increased risk for hypertension, type 2 diabetes mellitus, and atherosclerosis cardiovascular disease.

It is shocking to see children as young as 6 yrs old with diseases like hypertension, diabetes mellitus and abnormalities in the lipid profile, she said.

The study found that risk factor for Indian children for developing MS was at 70th WC percentile which is significantly lower than International proposed WC cut off of 90th percentile.

It also found that 3.3 percent or 358 children out of a total sample size of 10,842 were hypertensive.

"We found that primary or essential hypertension, commonly seen in adults, is becoming common in children, who are obese or overweight. Therefore it is very essential for them to change their lifestyle and lose weight," said Dr Anuradha Khadilkar, consultant paediatrician in Jehangir Hospital, Pune and corresponding author of the study.

The study will be published in the forthcoming issue of the Journal of Paediatrics.

Apr 29
Antibodies against deadly emerging disease MERS identified
Scientists have identified natural human antibodies against the virus that causes Middle East Respiratory Syndrome (MERS), a step toward developing treatments for the newly emerging and often-fatal disease.

The researchers found that these "neutralizing" antibodies prevented a key part of the virus, known as MERS CoV, from attaching to protein receptors that allow the virus to infect human cells. The research was led by Wayne Marasco , MD, an infectious disease expert at Dana-Farber.

Marasco and colleagues found the MERS antibodies using a "library" of some 27 billion human antibodies they have created and maintain in a freezer at Dana-Farber; it is one of the largest such libraries in the world.

Antibodies are proteins made by the immune system that recognize foreign viruses and bacteria. A neutralizing antibody is one that not only recognizes a specific virus but also prevents it from infecting host cells, so eventually the infection is "cleared" from the individual.

The research team plucked seven MERS-specific neutralizing antibodies from the library after using samples of the virus to screen for them.

MERS CoV (CoV stands for coronavirus) has on its surface an array of spike-shaped proteins that bind to host cells - specifically to receptor proteins called DPP4 on the surface of cells that line human airways. The neutralizing antibodies identified in the study prevented the virus' spikes from binding to the DPP4 receptors.

The researchers selected one of the antibodies, labeled 3B11, as a "lead" candidate for further research. Marasco said the antibody has been produced in sufficient quantities to begin testing in non-human primates and mice to determine if they protect against the virus. However these studies have been delayed because no good animal model for MERS has been developed, added Marasco.

The lab studies have been reported in the Proceedings of the National Academy of Sciences (PNAS).

Apr 29
Y chromosome's loss linked to shorter life expectancy and higher cancer risk for men
Researchers have shown a correlation between a loss of the Y chromosome in blood cells and both a shorter life span and higher mortality from cancer in other organs.

Men have a shorter average life span than women and both the incidence and mortality in cancer is higher in men than in women. However, the mechanisms and possible risk factors behind this sex-disparity are largely unknown. Alterations in DNA of normal cells accumulate throughout our lives and have been linked to diseases such as cancer and diabetes.

In a study recently published in the journal Nature Genetics an international team of researchers have analysed the DNA in blood samples from a group of more than 1,600 elderly men. They found that the most common genetic alteration was a loss of the Y chromosome in a proportion of the white blood cells.

The group of men was studied for many years and the researchers could detect a correlation between the loss of the Y chromosome and shorter survival.

Lars Forsberg , researcher at the Department of Immunology, Genetics and Pathology, Uppsala University, who has led the study, said men who had lost the Y chromosome in a large proportion of their blood cells had a lower survival, irrespective of cause of death. We could also detect a correlation between loss of the Y chromosome and risk of cancer mortality.

The Y chromosome is only present in men and the genes contained on the Y chromosome have so far mostly been associated with sex determination and sperm production.

The study has been recently published in the journal Nature Genetics.

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